Detection of thymidine kinase activity using an assay based on the precipitation of nucleoside monophosphates with lanthanum chloride
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1Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130.
Analytical Biochemistry
|April 1, 1989
Abstract:
A new assay method for the measurement of thymidine kinase (TK) is described. Cytosols were prepared from TK- and TK+ cells and evaluated for TK activity using an assay which is based on the phosphorylation of [125I]-iododeoxyuridine, [125I]-iododeoxycytidine, or [3H]thymidine and the precipitation of the monophosphates of these nucleosides by lanthanum chloride. The specificity, reproducibility, sensitivity, and convenience of this assay are demonstrated.