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Isolation of modified histone H3 from ultraviolet-irradiated deoxyribonucleoprotein by reversed-phase
L P Kurochkina1, Kolomijtseva GYa
1A. N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
Analytical Biochemistry
|April 1, 1989
Abstract:
A rapid reversed-phase high-performance liquid chromatography procedure for the isolation of histone H3 and/or of thymine modified at the lysine residue histone H3 from uv-irradiated deoxyribonucleoprotein and DNA-protein complex is reported. The system utilizes a C8 Ultrasphere macroporous column and an acetonitrile "inverse or negative gradient."