Related Experiment Video
Updated: Mar 19, 2026
12:17
Detection and Isolation of Apoptotic Bodies to High Purity
Published on: August 12, 2018
11.6K
Apoptosis in Cryopreserved Eukaryotic Cells
M A Savitskaya1, G E Onishchenko
1Lomonosov Moscow State University, Biological Faculty, Moscow, 119991, Russia. nakomis@mail.ru.
Biochemistry. Biokhimiia
|June 15, 2016
Summary
This review explores apoptosis mechanisms in cryopreserved cells, highlighting chemical agents that improve cell viability post-thaw. It emphasizes controlling oxidative stress with antioxidants to enhance cell survival during cryopreservation and thawing.
Area of Science:
- Cell biology
- Cryobiology
- Biochemistry
Background:
- Cryopreservation is essential for long-term cell storage but often induces cellular damage.
- Apoptosis, or programmed cell death, is a major challenge impacting cell viability after cryopreservation.
- Oxidative stress is a significant damaging factor during the cryopreservation and thawing processes.
Purpose of the Study:
- To review apoptosis mechanisms in cryopreserved cells.
- To discuss chemical agents that can control these mechanisms and improve cell viability.
- To evaluate the role of antioxidants in mitigating cryodamage.
Main Methods:
- Literature review of studies on apoptosis in cryopreserved cells.
- Analysis of chemical agents and their effects on cell viability.
- Examination of the impact of oxidative stress and antioxidants on cell survival.
Main Results:
- Specific apoptosis pathways are activated in cryopreserved cells.
- Chemical agents can modulate these pathways, enhancing post-thaw cell recovery.
- Antioxidants show promise in reducing oxidative stress and improving cell viability, both alone and in combination therapies.
Conclusions:
- Understanding apoptosis mechanisms is key to improving cryopreservation protocols.
- Targeted chemical interventions, particularly antioxidants, can significantly enhance cell viability after cryopreservation and thawing.
- Further research into combined antioxidant strategies may offer optimal cell protection.
More Related Videos
09:18Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death
Published on: December 27, 2016
9.2K
13:54Preparation of Cell-lines for Conditional Knockdown of Gene Expression and Measurement of the Knockdown Effects on E4orf4-Induced Cell Death
Published on: October 21, 2012
11.4K
Related Concept Videos
01:30Apoptosis
16.7K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
16.7K
01:30Overview of Cell Death
10.9K
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
10.9K
01:17The Extrinsic Apoptotic Pathway
9.2K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
9.2K
01:17Phagocytosis of Apoptotic Cells
5.5K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...
Normal cells contain receptors that prevent them from being recognized...
5.5K
01:18Autophagic Cell Death
4.9K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
4.9K
01:31The Intrinsic Apoptotic Pathway
9.1K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
9.1K