Cluster Analysis by Measurement of Peroxidase and Esterase from Citrus Flavedo
X Hong Zheng1,2, M Nishioka1, A Kawamura1
1a Department of Bioresources Science, Faculty of Agriculture , Kochi University , B-200 Monobe, Nankoku-shi, Kochi 783 , Japan.
Abstract:
The zymograms of peroxidase (POD) and esterase (EST) from seventy-two kinds of Citrus fruits and the fruit of both Poncirus and Fortunella were obtained by polyacrylamide gel electrophoresis. It was found that there was little variance in the band pattern of POD and EST of the fruits harvested after September. There were at least three bands of POD and approximately ten of EST in each cultivar. The density of each band for EST was measured with a densitometer. There was a characteristic band for POD identifying pummelos. The band patterns of EST were also characteristic among each species or cultivar. Two kinds of ootachibana (Citrus otachibana Hort. ex Tanaka) were shown to be different cultivars from each other according to their EST zymograms and peel oil analysis. Cluster analysis based on EST was done and discussed together with POD.
