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Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
Published on: July 14, 2015
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Three-dimensional ordered titanium dioxide-zirconium dioxide film-based microfluidic device for efficient on-chip
De Zhao1, Zhongyuan He1, Gang Wang1
1State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, PR China.
Journal of Colloid and Interface Science
|June 15, 2016
Summary
Microfluidic devices using titanium dioxide-zirconium dioxide inverse opal films efficiently enrich phosphopeptides. The best performance was achieved with the smallest pore size, demonstrating a promising approach for biomolecule separation.
Area of Science:
- Materials Science
- Biotechnology
- Analytical Chemistry
Background:
- Microfluidic technology offers miniaturized, automated platforms for biomolecule separation.
- Functional micro/nanostructured materials enhance microfluidic device performance and applications.
- Inverse opal structures, with their ordered 3D networks, large surface areas, and good mass transport, are suitable for bio-separation.
Purpose of the Study:
- To exploit inverse opal titanium dioxide-zirconium dioxide films for on-chip phosphopeptide enrichment.
- To evaluate the impact of inverse opal film structure on phosphopeptide enrichment efficiency.
Main Methods:
- Fabrication of microfluidic devices using titanium dioxide-zirconium dioxide inverse opal films derived from 270-, 340-, and 370-nm poly(methylmethacrylate) sphere templates.
- Assessment of phosphopeptide enrichment capabilities using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry.
Main Results:
- The device fabricated from the 270-nm sphere template demonstrated superior phosphopeptide enrichment compared to devices from larger templates.
- The smallest pore size and largest surface area of the inverse opal film, resulting from the smallest template, correlated with enhanced enrichment.
- The titanium dioxide-zirconium dioxide inverse opal film-based device outperformed similar devices utilizing nanoparticle or single-component films.
Conclusions:
- Titanium dioxide-zirconium dioxide inverse opal films are effective for on-chip phosphopeptide enrichment.
- Optimizing inverse opal structure, specifically pore size and surface area, is crucial for efficient biomolecule separation.
- These devices present a promising strategy for advanced biomolecule separation in microfluidic systems.
