Related Experiment Video
Updated: Mar 19, 2026
High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA
Published on: September 13, 2012
Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Protocols
Asunción García-Sánchez1,2, Fernando Marqués-García3,4
1Department of Biomedical and Diagnostic Sciences, University of Salamanca, Salamanca, Spain. chonela@usal.es.
Abstract:
The RNA interference (RNAi) plays an important role in regulation of gene expression. It is a mechanism used by many organisms to silence the expression of genes that control different processes in the cell. The double strand (ds) RNA molecule inhibits gene expression of a targeted gene with high specificity and selectivity.Different types of small ribonucleic acid molecules, microRNA (miRNA), small interfering RNA (siRNA), short hairpin RNA (shRNA), and the piwi RNA (piRNA) are involved in the RNA interference. RNAi is a relevant research tool in cell cultures and in vivo experiments because synthetic dsRNA introduced into cells can selectively silence specific target genes.Here, we describe a general guide for gene silencing mediated by siRNA, focusing on the most used delivery methods: lipid-mediated and electroporation transfection.
More Related Videos
15:55Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy
Published on: June 21, 2013
07:49CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
Published on: May 30, 2025
