Related Experiment Video
Updated: Mar 19, 2026
Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells
Published on: July 22, 2016
Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line
Elena Marcos-Vadillo1,2, Asunción García-Sánchez3,4
1Department of Clinical Biochemistry, University Hospital of Salmanca, Paseo de San Vicente 58, Salmanca, 37007, Spain. elemarcos@hotmail.com.
Abstract:
Trials of transfection in eukaryotic cells are essential tools for the study of gene and protein function. They have been used in a wide range of research fields. In this chapter, a method of transient transfection of the A549 cell line, human lung cells of alveolar epithelium, with an expression plasmid is described. In addition, the fundamental characteristics of this experimental procedure are addressed.
