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Published on: September 24, 2015
Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy
Alberto Rodríguez-Pulido1, Aitziber L Cortajarena2, Joaquim Torra3
1Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanoscience) and Nanobiotechnology Unit Associated to the National Center for Biotechnology (CSIC), 28049 Madrid, Spain. cristina.flors@imdea.org.
Abstract:
Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.

