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High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
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Exploring host-pathogen interactions through genome wide protein microarray analysis.

Luigi Scietti1, Katia Sampieri1, Irene Pinzuti1

  • 1GSK Vaccines, Via Fiorentina 1, 53100 Siena, Italy.

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|June 16, 2016
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Summary
This summary is machine-generated.

This study used protein microarrays to discover new interactions between human and bacterial proteins. Novel connections were found between Staphylococcus aureus and Neisseria meningitidis proteins and human targets, aiding in understanding pathogenesis.

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Area of Science:

  • Microbiology
  • Immunology
  • Proteomics

Background:

  • Bacterial pathogenesis involves extensive interactions between human and bacterial extracellular proteins.
  • Traditional methods for identifying these host-pathogen interactions are laborious and time-consuming.

Purpose of the Study:

  • To develop and apply a large-scale protein microarray-based screening approach to identify novel host-pathogen interactions.
  • To investigate interactions involving Staphylococcus aureus and Neisseria meningitidis.

Main Methods:

  • Utilized large libraries of human and bacterial recombinant proteins for screening.
  • Employed protein microarrays for high-throughput interaction analysis.
  • Screened human extracellular proteins against Staphylococcus aureus proteins and Neisseria meningitidis adhesin (NadA) against human proteins.

Main Results:

  • Identified a novel interaction between Staphylococcus aureus immune evasion protein FLIPr (formyl-peptide receptor like-1 inhibitory protein) and human complement component C1q.
  • Discovered an interaction between Neisseria meningitidis adhesin (NadA) and the human endothelial receptor LOX-1 (low-density oxidized lipoprotein receptor).

Conclusions:

  • The protein microarray approach efficiently identifies novel bacterial-human extracellular protein interactions.
  • These newly identified interactions offer insights into the molecular mechanisms of Staphylococcus aureus and Neisseria meningitidis pathogenesis.