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[Sensitivity of Pseudomonas mallei to sulfanilamide combinations in vitro]
Abstract:
It was shown that 9 strains of P. mallei were sensitive to sulfanilamides and their combinations with trimethoprim: sulfamonomethoxine + trimethoprim (2.5:1), sulfamethoxazole + trimethoprim (5:1) and sulfazin + trimethoprim (2.5:1 and 5:1). In regard to multiple lowering of the minimum bactericidal concentration of both drugs the combinations of sulfazin with trimethoprim (5:1 or 2.5:1) and sulfamethoxazole with trimethoprim (5:1) or biseptol proved to be the most active. The activity of the sulfamonomethoxine combination with trimethoprim (sulfate) was lower.
Insights
Nine strains of Pseudomonas mallei showed sensitivity to sulfanilamides combined with trimethoprim. Combinations of sulfazin and sulfamethoxazole with trimethoprim demonstrated the highest antibacterial activity against P. mallei.
Area of Science:
- Microbiology
- Pharmacology
Background:
- Pseudomonas mallei is a Gram-negative bacterium responsible for glanders.
- Antibiotic resistance in P. mallei necessitates the exploration of effective treatment combinations.
Purpose of the Study:
- To evaluate the in vitro efficacy of various sulfanilamide and trimethoprim combinations against P. mallei strains.
Main Methods:
- Testing the sensitivity of nine P. mallei strains to different sulfanilamide-trimethoprim ratios.
- Determining the minimum bactericidal concentration (MBC) for each drug combination.
Main Results:
- All tested P. mallei strains were sensitive to sulfanilamides and trimethoprim combinations.
- Sulfazin-trimethoprim (2.5:1 and 5:1) and sulfamethoxazole-trimethoprim (5:1) exhibited the most significant reduction in MBC.
- Sulfamonomethoxine-trimethoprim showed lower activity.
Conclusions:
- Sulfanilamide and trimethoprim combinations are effective against P. mallei in vitro.
- Specific ratios of sulfazin-trimethoprim and sulfamethoxazole-trimethoprim offer potent antibacterial activity, suggesting potential therapeutic applications.