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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Mar 19, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

Comprehensive phantom for interventional fluorescence molecular imaging.

Maria Anastasopoulou¹, Maximilian Koch¹, Dimitris Gorpas¹

¹Helmholtz Zentrum München, Institute for Biological and Medical Imaging, Ingolstädter Landstraße 1, Neuherberg D-85764 GermanybTechnical University Munich, Chair for Biological Imaging, Arcisstraße 21, Munich D-80333, Germany.

Journal of Biomedical Optics

|June 16, 2016

Summary

This summary is machine-generated.

Fluorescence imaging aids surgical guidance by visualizing disease biomarkers. New phantoms are crucial for calibrating these systems and improving cancer detection accuracy during operations.

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Last Updated: Mar 19, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

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Published on: July 17, 2012

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Area of Science:

Biomedical optics
Medical imaging
Surgical technology

Background:

Fluorescence imaging has been explored for surgical guidance for decades.
Disease-specific fluorescent agents improve intraoperative cancer identification over nonspecific dyes.
Advanced imaging necessitates robust calibration tools like phantoms.

Purpose of the Study:

To review progress in fluorescence phantoms for system validation.
To identify limitations in current phantom development.
To propose a phantom design for interlaboratory evaluation.

Main Methods:

Review of existing fluorescence phantom literature.
Analysis of requirements for advanced fluorescence imaging calibration.
Presentation of a novel phantom design.

Main Results:

Progress in fluorescence phantom development for system validation was reviewed.
Current limitations in phantom complexity and universality were identified.
A phantom design for interlaboratory system performance evaluation was proposed.

Conclusions:

Phantoms are essential for validating fluorescence imaging systems.
Developing complex phantoms is key to a universal calibration strategy.
The proposed phantom design facilitates interlaboratory comparisons.