Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hypoxia in brain slices.

K Shimoji1, H Higashi, N Fujiwara

  • 1Department of Anesthesiology, Nigata University School of Medicine, Japan.

Biomedica Biochimica Acta
|January 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surgically avertable burden of obstetric conditions in low- and middle-income regions: a modelled analysis.

BJOG : an international journal of obstetrics and gynaecology·2014
Same author

Prophylactic procedure for inguinal hernia after radical retropubic prostatectomy.

Hernia : the journal of hernias and abdominal wall surgery·2014
Same author

Isolation of a distinct class of gain-of-function SHP-2 mutants with oncogenic RAS-like transforming activity from solid tumors.

Oncogene·2008
Same author

Helicobacter pylori CagA interacts with E-cadherin and deregulates the beta-catenin signal that promotes intestinal transdifferentiation in gastric epithelial cells.

Oncogene·2007
Same author

MRI appearance of primary giant ovarian leiomyoma in a hysterectomised woman.

The British journal of radiology·2006
Same author

[Extracorporeal membrane oxygenation for severe respiratory failure during thoracic aortic aneurysm repair].

Kyobu geka. The Japanese journal of thoracic surgery·2006
Same journal

Intracerebroventricular administration of hypertonic sodium chloride solution reduces the sensitivity of the baroreceptor heart reflex in anaesthetized rats.

Biomedica biochimica acta·1991
Same journal

An efficient chemical-enzymatic synthesis of LHRH N-terminal pentapeptide.

Biomedica biochimica acta·1991
Same journal

Adrenergic cardiovascular actions in rats as affected by dichloromethane exposure.

Biomedica biochimica acta·1991
Same journal

Lipoxygenase-inhibitory action of antiviral polymeric oxidation products of polyphenols.

Biomedica biochimica acta·1991
Same journal

Uptake of lysine and incorporation into proteins of the cortex during a short-term hypoxia of neonatal rats.

Biomedica biochimica acta·1991
Same journal

Influence of intestinal resection, type of dietary fat and time on the digestive and metabolic utilization of fat in rats.

Biomedica biochimica acta·1991
See all related articles

Hypoxia in rat brain slices causes initial hyperpolarization then slow depolarization in hippocampal CA1 neurons. These changes affect neuronal membrane resistance and excitability, impacting synaptic transmission.

Area of Science:

  • Neuroscience
  • Cellular Physiology

Background:

  • Hypoxia, or oxygen deprivation, significantly impacts brain function.
  • Hippocampal CA1 neurons are crucial for learning and memory and are highly sensitive to oxygen levels.

Purpose of the Study:

  • To investigate the electrophysiological effects of hypoxia on rat hippocampal CA1 neurons in vitro.
  • To characterize the membrane potential and resistance changes during hypoxic conditions.

Main Methods:

  • Intracellular recording from rat hippocampal CA1 neurons in brain slices.
  • Superfusion with hypoxic (95%N2-5% CO2) and normoxic media.
  • Application of ouabain and manipulation of extracellular potassium concentrations.

Main Results:

Related Experiment Videos

  • Hypoxia induced a transient depolarization followed by hyperpolarization and then a slow depolarization.
  • Both hyperpolarization and depolarization were linked to reduced membrane resistance.
  • Excitatory postsynaptic potentials (EPSPs) were preserved longer than inhibitory postsynaptic potentials (IPSPs) under hypoxia.
  • Neuronal spikes were maintained but with increased threshold and decreased amplitude.

    • Conclusions:

    • Hypoxia profoundly alters hippocampal CA1 neuron electrophysiology, affecting membrane potential and resistance.
    • These changes disrupt synaptic transmission, with differential effects on excitatory and inhibitory pathways.
    • The findings provide insights into neuronal vulnerability during oxygen deprivation.