Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

3.5K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
3.5K
Adult Stem Cells01:33

Adult Stem Cells

34.2K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
34.2K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.6K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.6K
Stem Cell Niche01:26

Stem Cell Niche

6.5K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
6.5K
Multipotency and Niche of Bulge Stem Cell01:06

Multipotency and Niche of Bulge Stem Cell

4.3K
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
4.3K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.8K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PHLDA1 Mediates Drug Resistance in Receptor Tyrosine Kinase-Driven Cancer.

Cell reports·2026
Same author

Gene mutant dosage is associated with prognosis and metastatic tropism in 60,000 clinical cancer samples.

medRxiv : the preprint server for health sciences·2026
Same author

Spatially resolved transcriptomic and proteomic profiling reveals cell interaction programs that predict Barrett's esophagus progression.

bioRxiv : the preprint server for biology·2026
Same author

PHYFUM: Phylogenetic Reconstruction of Normal and Pre-malignant Tissue Evolution Using Fluctuating Methylation.

bioRxiv : the preprint server for biology·2026
Same author

Integrating artificial intelligence (AI) into colorectal cancer reporting.

The Journal of pathology·2026
Same author

Negative Selection Maintains Grossly Altered but Broadly Stable Karyotypes in Metastatic Colorectal Cancer.

Cancer discovery·2026
Same journal

Mutation detection in women diagnosed with endometrial cancer: a next-generation sequencing analysis.

Molecular & cellular oncology·2026
Same journal

Evolutionary perspectives on endometrial cancer: antagonistic pleiotropy.

Molecular & cellular oncology·2026
Same journal

mRNA vaccines in oncology: personalized cancer immunization and neoantigen targeting.

Molecular & cellular oncology·2026
Same journal

Exploration of the diagnostic and therapeutic potential of the nucleocytoplasmic shuttling protein TMUB1 by inducing G0/G1 cell cycle arrest in ovarian cancer.

Molecular & cellular oncology·2026
Same journal

The LNK adaptor protein: a dual regulator of proliferation and migration in solid tumors.

Molecular & cellular oncology·2026
Same journal

Lactylation-related genes signature panel in hepatocellular carcinoma reveals the prognostic and therapeutic optimization.

Molecular & cellular oncology·2026
See all related articles

Related Experiment Video

Updated: Mar 19, 2026

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
07:46

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

Published on: October 11, 2022

4.1K

Revealing human intestinal stem cell and crypt dynamics.

Ann-Marie Baker1, Trevor A Graham1

  • 1Evolution and Cancer Laboratory; Barts Cancer Institute; Barts and the London School of Medicine and Dentistry; Queen Mary University of London ; London, UK.

Molecular & Cellular Oncology
|June 17, 2016
PubMed
Summary
This summary is machine-generated.

Human intestinal stem cell dynamics were poorly understood. This study used somatic mutations and mathematical modeling to reveal the in vivo temporal dynamics of these crucial gut stem cells.

Keywords:
clonal evolutioncrypt life-cyclehuman intestineneutral driftstem cell

More Related Videos

Laser Ablation and Intravital Microscopy to Study Intestinal Remodeling
09:42

Laser Ablation and Intravital Microscopy to Study Intestinal Remodeling

Published on: June 9, 2023

2.1K
Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
07:42

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Published on: July 13, 2016

74.0K

Related Experiment Videos

Last Updated: Mar 19, 2026

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
07:46

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

Published on: October 11, 2022

4.1K
Laser Ablation and Intravital Microscopy to Study Intestinal Remodeling
09:42

Laser Ablation and Intravital Microscopy to Study Intestinal Remodeling

Published on: June 9, 2023

2.1K
Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
07:42

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Published on: July 13, 2016

74.0K

Area of Science:

  • Gastroenterology
  • Stem Cell Biology
  • Computational Biology

Background:

  • Human intestinal stem cell dynamics are not well understood.
  • Characterizing these dynamics is crucial for understanding gut homeostasis and disease.

Purpose of the Study:

  • To investigate the in vivo temporal dynamics of human intestinal stem cells.
  • To address the poor characterization of stem cell and crypt dynamics in the human gut.

Main Methods:

  • Utilized random somatic mutations as endogenous barcodes to trace stem cell lineages in the human intestine.
  • Employed mathematical modeling to analyze mutation data and infer stem cell dynamics.

Main Results:

  • Successfully traced stem cell lineages using somatic mutations.
  • Inferred the in vivo temporal dynamics of human intestinal stem cells through mathematical modeling.

Conclusions:

  • Provided novel insights into the dynamics of human intestinal stem cells.
  • Established a framework for studying stem cell behavior in the human gut using endogenous markers and computational approaches.