Related Experiment Video

Updated: Mar 19, 2026

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
07:26

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

1.1K

Sonic Hedgehog factor Gli1: As good as resistant

Hiba Ahmad Zahreddine1, Biljana Culjkovic-Kraljacic1, Katherine Lb Borden1

  • 1Institute for Research in Immunology and Cancer; University of Montreal ; Montreal, QC Canada.

Molecular & Cellular Oncology
|June 17, 2016
PubMed

Insights

Chemoresistance is a major hurdle in cancer treatment. Our research shows Gli1 protein activates drug metabolism enzymes, leading to a new understanding of how cancer cells resist chemotherapy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Chemoresistance significantly hinders effective cancer treatment.
  • Understanding resistance mechanisms is crucial for developing new therapies.
  • The role of Gli1 in drug resistance is not fully understood.

Purpose of the Study:

  • To investigate the role of Gli1 in chemoresistance.
  • To identify novel mechanisms of drug resistance in cancer.
  • To explore the involvement of Phase II drug metabolism enzymes in Gli1-mediated resistance.

Main Methods:

  • Utilized molecular biology techniques to study Gli1 expression and function.
  • Investigated the impact of Gli1 on the expression and activity of Phase II drug metabolism enzymes.
  • Analyzed the effect of Gli1 modulation on cancer cell sensitivity to chemotherapeutic agents.

Main Results:

  • Provided evidence that Gli1 promotes chemoresistance.
  • Identified a novel mechanism of drug resistance driven by Gli1.
  • Demonstrated that Gli1 activates specific Phase II drug metabolism enzymes, particularly the UGT1A family.

Conclusions:

  • Gli1 plays a significant role in driving chemoresistance.
  • Activation of UGT1A family enzymes by Gli1 represents a novel resistance pathway.
  • Targeting Gli1 or UGT1A enzymes may offer new therapeutic strategies to overcome chemoresistance.
Keywords:
Gli1UGT1AVismodegibcancercytarabinedrug resistanceeIF4Eglucuronidationribavirin

More Related Videos

Triggering Reactive Gliosis In Vivo by a Forebrain Stab Injury07:46

Triggering Reactive Gliosis In Vivo by a Forebrain Stab Injury

Published on: June 29, 2015

11.1K
Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells06:32

Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells

Published on: September 1, 2010

16.9K

Related Concept Videos

Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
10.3K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

1.8K
Resistance and Conductance01:25

Resistance and Conductance

A conductor's DC resistance at a given temperature is influenced by its resistivity, length, and cross-sectional area. Resistivity is an inherent property of the conductor material, with annealed copper serving as the international standard for measurement. For instance, the resistivity of hard-drawn aluminum at 20 degrees Celsius is 61% of the standard conductivity of annealed copper.
Various factors impact the resistance of a conductor. Spiraling in stranded conductors increases their...
579