The Relative Functionality of Freshly Isolated and Cryopreserved Human Adipose-Derived Stromal/Stem Cells
Forum S Shah1, Jie Li, Fabiana Zanata
1LaCell LLC, Tulane University, New Orleans, La., USA.
Cells, Tissues, Organs
|June 17, 2016
Summary
Cryopreserved human adipose-derived stromal/stem cells (ASCs) maintain key characteristics after storage. However, their differentiation capacity is reduced, indicating a need for improved cryopreservation methods for therapeutic applications.
Area of Science:
- Stem Cell Biology
- Regenerative Medicine
- Cryobiology
Background:
- Mesenchymal stem cells (MSCs) are crucial for therapeutic applications.
- Banking and cryopreservation of MSCs are essential for future use.
- Assessing the impact of cryopreservation on cell properties is vital.
Purpose of the Study:
- To evaluate the effect of long-term cryostorage (9-10 months) on human adipose-derived stromal/stem cells (ASCs).
- To assess changes in morphology, immunophenotype, colony-forming unit (CFU) frequency, and differentiation capacity of cryopreserved ASCs compared to fresh cells.
Main Methods:
- Human ASCs from the same donors were analyzed fresh and after 9-10 months of cryopreservation.
- Morphology, immunophenotype (CD29, CD73, CD90, CD105, CD34, CD45), CFU frequency, and differentiation potential were assessed.
Main Results:
- Cryopreservation did not significantly alter CFU frequency.
- Expression of key MSC markers (CD29, CD73, CD90, CD105) remained stable.
- A significant reduction in differentiation capacity was observed in cryopreserved ASCs compared to fresh cells.
Conclusions:
- Cryopreserved ASCs retain essential characteristics for research and clinical utility.
- Further research is needed to optimize cryopreservation techniques to preserve differentiation potential.
- Long-term cryostorage impacts ASC differentiation capacity, necessitating method improvements.
