Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

4.8K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
4.8K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

2.8K
2.8K
Electrochemical Gradient and Channel Proteins: An Overview01:21

Electrochemical Gradient and Channel Proteins: An Overview

5.2K
An electrochemical gradient is a fundamental concept in biology and chemistry. It regulates the movement of ions across cell membranes. This movement is influenced by two factors:
The electrical gradient: The electrical gradient across cell membranes refers to the difference in electric charge between the inside and outside of a cell.  This difference drives the movement of ions towards or away from the cells. For instance, if the inside of the cell is more negatively charged relative to...
5.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surface-Density-Controlled Spreading-Packing Competition in Antibody Monolayers Revealed by PM-IRRAS and 2D Correlation Spectroscopy.

ACS omega·2026
Same author

Cumulative Bisphenol A Release and Elution Kinetics from Pediatric Restorative and Orthodontic Resin-Based Materials: An In Vitro LC-MS/MS Investigation.

Children (Basel, Switzerland)·2026
Same author

Cyanoacrylate Tissue Adhesives in Oral and Maxillofacial Surgery: A Narrative Review of Clinical Outcomes.

Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons·2026
Same author

Leveraging multitargeting BChE-MAO B inhibitors against microglia-related neuroinflammation: in vitro biological evaluation, structure-activity relationships, drug-like properties, and X-ray crystal complexes.

European journal of medicinal chemistry·2026
Same author

MD Simulations of Human Sigma-1 Receptor Trimer Uncover Cholesterol-Dependent Stabilization and Ligand-Specific Dynamics.

Journal of chemical information and modeling·2026
Same author

Radioactivity behavior in hydrothermal volcanic systems: Preliminary in situ investigations at high and low gas flux degassing areas.

Journal of environmental radioactivity·2026

Related Experiment Video

Updated: Mar 19, 2026

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
11:55

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling

Published on: May 29, 2011

15.6K

Organic bioelectronics probing conformational changes in surface confined proteins.

Eleonora Macchia1, Domenico Alberga2, Kyriaki Manoli1

  • 1Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro - Bari (Italy).

Scientific Reports
|June 18, 2016
PubMed
Summary

This study integrates biotin-binding proteins into organic thin-film transistors (TFTs) to analyze protein-ligand interactions. The research distinguishes changes in protein morphology and dipole moment, advancing bio-electronic platform development.

More Related Videos

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

8.3K
Translating Extracellular Electron Transfer Activities with Organic Electrochemical Transistors
10:44

Translating Extracellular Electron Transfer Activities with Organic Electrochemical Transistors

Published on: January 31, 2025

1.5K

Related Experiment Videos

Last Updated: Mar 19, 2026

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
11:55

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling

Published on: May 29, 2011

15.6K
Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
08:46

Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis

Published on: September 16, 2014

8.3K
Translating Extracellular Electron Transfer Activities with Organic Electrochemical Transistors
10:44

Translating Extracellular Electron Transfer Activities with Organic Electrochemical Transistors

Published on: January 31, 2025

1.5K

Area of Science:

  • Bio-electronics
  • Protein-surface interactions
  • Organic electronics

Background:

  • Protein-surface interactions are crucial for developing bio-systems.
  • Organic bio-electronic platforms offer insights into protein interface processes.

Purpose of the Study:

  • To investigate protein-ligand complex changes using organic thin-film transistors (TFTs).
  • To decouple and analyze alterations in protein morphology and dipole moment upon ligand binding.

Main Methods:

  • Integration of biotin-binding proteins into two distinct organic TFT configurations.
  • Analysis of output current changes, threshold voltage, and field-effect mobility.
  • Molecular Dynamics simulations of avidin tetramer with and without ligands.

Main Results:

  • Threshold voltage changes correlate with protein dipole moment alterations.
  • Field-effect mobility changes indicate protein conformational shifts upon ligand binding.
  • Molecular Dynamics simulations support experimental findings on ligand-induced changes.

Conclusions:

  • Organic TFTs can effectively probe protein-ligand interactions by analyzing distinct electrical parameters.
  • The study provides a comprehensive understanding of how ligand binding affects protein dipole moments and conformations.
  • This work contributes to the advancement of bio-electronic platforms for studying biological interfaces.