Related Experiment Video
Updated: Mar 19, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
Published on: September 7, 2017
The second hit of DNA methylation.
Annalisa Di Ruscio1, Robert S Welner2, Daniel G Tenen3
1Harvard Medical School, Boston, MA, USA; Harvard Stem Cell Institute, Boston, MA, USA; Department of Translational Medicine, University of Eastern Piedmont "Amedeo Avogadro", Novara, Italy.
Epigenetic information, stored as DNA and histone modifications, regulates gene expression. New technologies allow analysis of epigenetics' role in human cancer.
Area of Science:
- Molecular Biology
- Genetics
- Cancer Research
Background:
- Gene expression is controlled by heritable epigenetic information.
- Epigenetic information includes chemical modifications of DNA and histones.
- Understanding epigenetics is crucial for cancer research.
Purpose of the Study:
- To analyze the epigenetic contribution to human cancer.
- To leverage recent technological advancements in epigenome mapping and cancer cell reprogramming.
Main Methods:
- Utilizing sequencing-based epigenome mapping technologies.
- Employing cancer cellular reprogramming techniques.
Main Results:
- The study provides tools to analyze the epigenetic contribution to human cancer.
- Established methodologies for studying epigenetic alterations in cancer.
Conclusions:
- Epigenetic modifications play a significant role in human cancer.
- Technological advancements facilitate the investigation of epigenetic factors in cancer etiology and progression.
More Related Videos
06:07Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
Published on: August 5, 2022
13:47Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
Published on: February 24, 2015
Related Concept Videos
Epigenetic Regulation
X-chromosome...
Epigenetic Regulation
Epigenetic Regulation
Phase II Reactions: Methylation Reactions
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...
Histone Modification
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Histone Modification