Related Experiment Video
Updated: Mar 19, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Thermophilic Alkaline Xylanase from Newly Isolated Alkaliphilic and Thermophilic Bacillus sp. Strain TAR-1
S Nakamura1, R Nakai1, K Wakabayashi1
1a Department of Bioengineering , Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology , 4259 Nagatsuta, Midori-ku, Yokohama 227 , Japan.
Abstract:
Alkaliphilic and thermophilic Bacillus sp. strain TAR-1, isolated from soil, produced a xylanase extracellularly. The xylanase was most active over a pH range of 5.0 to 9.5 at 50°C. Optimum temperatures of the crude xylanase preparation were 75°C at pH 7.0 and 70°C at pH 9.0. Zymogram analyses of the culture supernatant showed that the molecular mass of the xylanase was 40 kDa and the isoelectric point was pH 4.1. The predominant products of xylan hydrolysate were xylobiose, xylotriose, and higher oligosaccharides, indicating that the enzyme was an endoxylanase. Production of the thermophilic alkaline xylanase was induced by xylan and xylose, but was repressed in the presence of glucose.
Related Concept Videos
Hyperthermophilic Bacteria
Diversity of Archaea I
Diversity of Archaea IV
Anoxygenic Phototrophic Bacteria
Diversity of Archaea III
Bacterial Phylum Actinobacteria

