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Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

LTR Retrotransposons

LTR Retrotransposons

LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...

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Related Experiment Video

Updated: Mar 19, 2026

Author Spotlight: Exploring Macrophage Immunometabolism Through Lentiviral Vector-Mediated Gene Manipulation

Author Spotlight: Exploring Macrophage Immunometabolism Through Lentiviral Vector-Mediated Gene Manipulation

Published on: February 16, 2024

Conditional RNAi Using the Lentiviral GLTR System.

Elisabeth Pfeiffenberger¹, Reinhard Sigl¹, Stephan Geley²

¹Division of Molecular Pathophysiology, Biocenter, Innsbruck Medical University, Innrain 80/82, Innsbruck, Austria.

Methods in Molecular Biology (Clifton, N.J.)

|June 19, 2016

Summary

This summary is machine-generated.

This study introduces a novel lentiviral RNA interference (RNAi) system for creating stable cell lines with inducible RNA interference (RNAi). This technology enables rapid generation of cell lines for functional gene analysis.

Keywords:

GATEWAY Human cell line Inducible Knockdown Lentivirus RNAi Tetracycline shRNA

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Lentivirus Production

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Published on: October 2, 2009

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Lentivirus Production

Lentivirus Production

Published on: October 2, 2009

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

RNA interference (RNAi) is crucial for gene function studies.
Effective RNAi relies on expressing specific small interfering RNA molecules (siRNAs).

Purpose of the Study:

To present a new lentiviral RNAi system for inducible RNA interference (RNAi).
To enable rapid generation of stable cell lines for functional gene analysis.

Main Methods:

Utilized lentiviral vectors for conditional expression of short hairpin RNA (shRNA).
Employed GATEWAY recombination cloning for rapid vector generation.
Developed both one-vector and two-vector systems for conditional cell line establishment.

Main Results:

Demonstrated rapid generation of stable cell lines with inducible RNAi.
Showcased lentiviral vectors co-expressing fluorescent proteins (pGLTR-FP) for monitoring or combinatorial RNAi.
Included vectors for cell selection (pGLTR-S) and a one-vector system (pGLTR-X).

Conclusions:

The described lentiviral RNAi system facilitates efficient and inducible gene knockdown.
This technology accelerates the creation of cell lines for complex genetic studies.