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Analysis of D2d: a D-region class I gene
M L Hedley1, S W Hunt, K A Brorson
1University of Texas Southwestern Medical Center, Dallas 75235.
Immunogenetics
|January 1, 1989
Summary
The mouse D2d gene shares structural similarities with other class I genes but has a frameshift mutation. Despite low-level expression, D2d may function as a class I gene, possibly originating from the Qa region.
Area of Science:
- Immunogenetics
- Molecular Biology
- Mouse Genetics
Background:
- The mouse major histocompatibility complex (MHC) comprises K, D, Qa, and Tla regions.
- The D region in BALB/c mice contains D2d, D3d, D4d, H-2Dd, and H-2Ld genes.
Purpose of the Study:
- To determine the DNA sequence of the D2d gene.
- To analyze its exon/intron structure and regulatory elements.
- To investigate the surface expression of the D2d molecule.
Main Methods:
- DNA sequencing of the D2d gene.
- Comparison with known class I gene sequences.
- Generation of an exon-shuffled construct for transfection.
- Detection of cell surface protein expression using monoclonal antibodies.
Main Results:
- The D2d gene exhibits an exon/intron structure and 5' regulatory elements similar to other class I genes.
- A frameshift mutation in exon seven leads to a truncated cytoplasmic tail.
- Transfected mouse L cells expressed a D2d protein at the cell surface, detectable by specific antibodies.
Conclusions:
- The D2d gene is structurally similar to functional class I genes.
- Despite a frameshift mutation and low expression, D2d may be a functional class I gene.
- D2d likely evolved from a gene in the Qa region.