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The LORE1 insertion mutant resource.

Anna Małolepszy¹, Terry Mun¹, Niels Sandal¹

¹Centre for Carbohydrate Recognition and Signalling, Department of Molecular Biology and Genetics, Aarhus University, Gustav Wieds Vej 10, DK-8000, Aarhus C, Denmark.

The Plant Journal : for Cell and Molecular Biology

|June 21, 2016

Summary

This summary is machine-generated.

Researchers activated the Lotus retrotransposon 1 (LORE1) in Lotus japonicus, creating over 640,000 new mutations. This cost-efficient method generates non-transgenic plant mutants for research.

Keywords:

Lotus japonicus DNA methylation Long terminal repeat retrotransposon mutagenesis palindrome

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Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

Area of Science:

Genomics
Molecular Biology
Plant Science

Background:

Long terminal repeat (LTR) retrotransposons are mobile genetic elements related to retroviruses that significantly influence eukaryotic genome structure and evolution.
Understanding LTR retrotransposon insertion preferences is crucial for their application in targeted mutagenesis and functional genomics.

Purpose of the Study:

To generate a comprehensive insertion mutant collection in Lotus japonicus using the LTR element Lotus retrotransposon 1 (LORE1).
To analyze LTR retrotransposon insertion site characteristics and preferences for effective gene targeting.
To evaluate the potential of endogenous LTR retrotransposons for cost-efficient, non-transgenic mutant generation in plants.

Main Methods:

De novo activation of the LORE1 retrotransposon in a large population of Lotus japonicus.
Identification and analysis of over 640,000 LORE1 insertion events.
Characterization of insertion site motifs and preferences, including analysis of DNA methylation patterns.

Main Results:

A complete Lotus japonicus insertion mutant collection with 640,653 new LORE1 insertion events was generated.
The study identified a short, relaxed insertion site motif and inferred the mechanism for consensus palindrome generation at insertion sites.
Selective integration into CHG-hypomethylated genes was observed, leading to a high mutation rate and near saturation of active gene targeting within the population.
LORE1 active gene targeting approached saturation in 134,682 Lotus japonicus lines.

Conclusions:

Endogenous LTR retrotransposons with germinal activity, like LORE1, offer a general and cost-efficient strategy for creating non-transgenic mutant collections.
This approach facilitates unrestricted use of mutant collections in plant research, accelerating functional genomics studies.
The findings provide insights into retrotransposon insertion mechanisms and their impact on genome evolution.