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Related Experiment Video

Updated: Mar 19, 2026

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
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Current progress in genetically encoded voltage indicators for neural activity recording.

Shigenori Inagaki1, Takeharu Nagai2

  • 1Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan.

Current Opinion in Chemical Biology
|June 21, 2016
PubMed
Summary
This summary is machine-generated.

Genetically Encoded Voltage Indicators (GEVIs) offer superior spatiotemporal resolution for neural activity compared to fMRI and EEG. This review guides neuroscientists in selecting optimal GEVIs for diverse research applications.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biotechnology

Background:

  • Genetically Encoded Voltage Indicators (GEVIs) are crucial for studying neural activity.
  • GEVIs provide high spatiotemporal resolution, surpassing functional magnetic resonance imaging (fMRI) and electroencephalogram (EEG).
  • GEVIs enable simultaneous recording from hundreds of neurons, exceeding patch-clamp limitations.

Purpose of the Study:

  • To summarize the characteristics of available GEVIs.
  • To categorize GEVIs based on their voltage sensing mechanisms.
  • To provide a selection guideline for optimal GEVI use in neuroscience.

Main Methods:

  • Review of current Genetically Encoded Voltage Indicators.
  • Analysis of voltage sensing mechanisms.
  • Comparative assessment of GEVI performance.

Main Results:

  • GEVIs offer subcellular and millisecond resolution for neural activity.
  • GEVIs significantly outperform fMRI and EEG in spatiotemporal detail.
  • GEVIs allow simultaneous monitoring of hundreds of neurons versus tens with patch-clamp.

Conclusions:

  • Understanding GEVI pros and cons is vital for neuroscientists.
  • A clear guideline for selecting appropriate GEVIs is provided.
  • Optimal GEVI selection enhances neural activity investigations.