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Related Experiment Video

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Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
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Multidimensional colorimetric sensor array for discrimination of proteins.

Jinpeng Mao1, Yuexiang Lu2, Ning Chang1

  • 1Department of Chemistry, Capital Normal University, Beijing 100048, PR China.

Biosensors & Bioelectronics
|June 21, 2016
PubMed
Summary
This summary is machine-generated.

A new colorimetric sensor array using DNA-functionalized gold nanoparticles (DNA-AuNPs) can detect and differentiate proteins. This naked-eye detectable system shows high accuracy, even in human urine, offering potential for real-world biological fluid analysis.

Keywords:
Colorimetric sensor arrayDNA-AuNPsMultidimensional transduction principlesPattern recognitionProtein discrimination

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Area of Science:

  • Nanotechnology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Protein detection is crucial in diagnostics and research.
  • Existing methods can be complex and costly.
  • Developing sensitive, accessible detection platforms is essential.

Purpose of the Study:

  • To develop an extensible multidimensional colorimetric sensor array for protein detection.
  • To utilize DNA-functionalized gold nanoparticles (DNA-AuNPs) as versatile receptors.
  • To enable naked-eye discrimination of proteins and their conformational changes.

Main Methods:

  • Employing DNA-AuNPs whose aggregation and morphology change in response to different proteins and salt concentrations.
  • Utilizing a multidimensional transduction principle based on plasmonic nanoparticle conjugates.
  • Applying hierarchical cluster analysis (HCA) for data interpretation.

Main Results:

  • The sensor array accurately discriminated ten native proteins and their denatured conformations at 50 nM with 100% accuracy.
  • Different protein concentrations induced distinct color changes and nanoparticle morphologies.
  • The system demonstrated successful protein detection in 50% human urine at 500 nM concentration.

Conclusions:

  • The developed sensor array offers a rapid, simple, low-cost, and in-field method for protein detection.
  • This platform has significant potential for analyzing real biological fluids and investigating biomacromolecular conformational changes.
  • The multidimensional colorimetric approach provides a new direction for plasmonic nanoparticle-based sensing.