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Related Experiment Videos

Murine complement component 3: genetic variation and linkage to H-2.

F P Da Silva, G F Hoecker, N K Day

    Proceedings of the National Academy of Sciences of the United States of America
    |February 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

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    Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2006

    Researchers identified two forms of mouse complement component 3 (C3) using electrophoresis. Most strains had the slow (S) variant, while a few had the fast (F) variant, with inheritance following Mendelian patterns and linkage to H-2.

    Area of Science:

    • Immunogenetics
    • Molecular Biology
    • Biochemistry

    Background:

    • Complement component 3 (C3) is a crucial protein in the immune system.
    • Genetic variations in complement proteins can impact immune responses.
    • Understanding C3 polymorphism in mice is important for immunological research.

    Purpose of the Study:

    • To identify and characterize electrophoretic variants of murine complement component 3 (C3).
    • To determine the inheritance pattern of C3 variants in inbred and outbred mouse strains.
    • To investigate the genetic linkage between C3 variants and the H-2 major histocompatibility complex.

    Main Methods:

    • High-voltage electrophoresis of fresh mouse serum in agarose gels.
    • Analysis of C3 phenotypes (S, F, SF) in 46 inbred mouse strains and Swiss-Webster mice.

    Related Experiment Videos

  • Mendelian segregation analysis in controlled crosses and recombination frequency calculation with H-2.

    • Main Results:

    • Two electrophoretic variants of murine C3, designated S (slow) and F (fast), were identified.
    • The S allele was predominant in most inbred strains, while the F allele was found in a minority.
    • Inheritance of C3 variants followed Mendelian laws, with evidence of genetic linkage to the H-2 locus (recombination frequency ~0.12).

    Conclusions:

    • Murine C3 exhibits genetic polymorphism with two common electrophoretic variants.
    • The gene for C3 is located near the H-2 complex on mouse chromosome 17.
    • This C3 polymorphism provides a useful genetic marker for immunological studies in mice.