Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of Time of Day on Dry Eye Disease: Findings from a Large Dry Eye Clinic Cohort.

Clinical ophthalmology (Auckland, N.Z.)·2026
Same author

Corrigendum.

Ophthalmology·2026
Same author

Cytokine levels and clinical characteristics in the tear fluid of patients with atopic dermatitis and atopic keratoconjunctivitis.

The ocular surface·2026
Same author

Altered tear fluid protein expression in persons with mild cognitive impairment.

Journal of Alzheimer's disease : JAD·2026
Same author

Tele-Ophthalmology for Retinal Health Surveillance in Denmark.

Ophthalmology and therapy·2026
Same author

Minimizing Postoperative Scars in Epicanthoplasty: A Concise Review.

Journal of cosmetic dermatology·2025

Related Experiment Video

Updated: Mar 19, 2026

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging
09:56

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

7.1K

Nucleus Morphometry in Cultured Epithelial Cells Correlates with Phenotype.

Ayyad Z Khan1, Tor P Utheim2, Catherine J Jackson2

  • 11Institute of Clinical Medicine, Faculty of Medicine,University of Oslo,P.O Box 1171,Blindern,0318 Oslo,Norway.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|June 23, 2016
PubMed
Summary

Nuclear size in cultured epithelial cells correlates with proliferation markers, offering a potential non-invasive method for assessing cell phenotype before transplantation. However, the nucleus-to-cytoplasm ratio is not a reliable indicator across all cell types.

Keywords:
HCjEHEKN/C ratioRPEregenerative medicine

More Related Videos

Author Spotlight: Advancing 3D Coculture Systems with PVA-PCL Nanofibrous Membranes
10:08

Author Spotlight: Advancing 3D Coculture Systems with PVA-PCL Nanofibrous Membranes

Published on: December 27, 2024

911
Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures
09:26

Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures

Published on: June 27, 2022

3.1K

Related Experiment Videos

Last Updated: Mar 19, 2026

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging
09:56

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Published on: April 30, 2019

7.1K
Author Spotlight: Advancing 3D Coculture Systems with PVA-PCL Nanofibrous Membranes
10:08

Author Spotlight: Advancing 3D Coculture Systems with PVA-PCL Nanofibrous Membranes

Published on: December 27, 2024

911
Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures
09:26

Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures

Published on: June 27, 2022

3.1K

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Regenerative Medicine

Background:

  • The phenotype of cultured ocular epithelial cells impacts corneal transplant success.
  • Determining cell phenotype non-invasively before transplantation is crucial for clinical outcomes.

Purpose of the Study:

  • To investigate the relationship between nucleus morphometry and phenotype in three types of cultured epithelial cells.
  • To explore the potential of nucleus morphometry as a non-invasive method for assessing cell phenotype.

Main Methods:

  • Quantitative immunofluorescence was used to analyze cultured human conjunctival epithelial cells (HCjE), human epidermal keratinocytes (HEK), and human retinal pigment epithelial cells (HRPE).
  • Nucleus morphometry and nucleus-to-cytoplasm (N/C) ratio were assessed using ImageJ.
  • Spearman's correlation coefficient was employed for statistical analysis.

Main Results:

  • Nuclear area positively correlated with the proliferation marker PCNA in all three cell types.
  • Nuclear area showed significant correlations with undifferentiated cell markers (ABCG2 in HCjE, Bmi1 in HEK).
  • The N/C ratio did not consistently correlate with phenotype markers across HCjE, HEK, and HRPE.

Conclusions:

  • Nuclear size is a promising morphometric indicator for assessing the phenotype of cultured epithelia.
  • The N/C ratio is not a universally applicable marker for in vitro phenotype assessment in these cell types.
  • These findings support the development of non-invasive methods for pre-transplantation evaluation of ocular epithelial cells.