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Synthesis of a fluorescence resonance energy transfer-based probe containing a tricyclic nucleoside analog for single

Aya Hayai¹, Yusuke Maeda¹, Yoshihito Ueno²

¹Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.

Bioorganic & Medicinal Chemistry Letters

|June 23, 2016

Abstract:

Here, we report the synthesis of a fluorescence resonance energy transfer (FRET)-based probe for single nucleotide polymorphism (SNP) typing. The probe contains a fluorescent tricyclic base, 8-amino-3-(2,3-dihydroxypropyl)imidazo[4',5':5,6]pyrido[2,3-d]pyrimidine, as a donor molecule and 7-diethylaminocoumarin-3-carboxylic acid as an acceptor molecule. FRET was observed between the donor and acceptor molecules on the probe. The identity of the target bases on DNA and RNA strands could be determined using the probe.

Keywords:

DNA FRET Fluorescence Probe RNA SNPs

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