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Updated: Mar 19, 2026
Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography
Published on: March 10, 2023
Comprehensive analysis of high-throughput screens with HiTSeekR.
Markus List1, Steffen Schmidt2, Helle Christiansen2
1Lundbeckfonden Center of Excellence in Nanomedicine (NanoCAN), University of Southern Denmark, 5000 Odense, Denmark Molecular Oncology, Institute of Molecular Medicin (IMM), University of Southern Denmark, 5000 Odense, Denmark Clinical Institute (CI), University of Southern Denmark, 5000 Odense, Denmark markus.list@mpi-inf.mpg.de.
We developed HiTSeekR, a user-friendly software for analyzing high-throughput screening (HTS) data. This tool aids in identifying drug targets and interpreting results within systems biology for various screen types.
Area of Science:
- Computational Biology
- Genomics
- Drug Discovery
Background:
- High-throughput screening (HTS) is crucial for drug discovery but lacks integrated, user-friendly software for data analysis and interpretation.
- Existing tools often fail to robustly identify hits or connect findings to systems biology contexts.
Purpose of the Study:
- To develop HiTSeekR, a comprehensive software solution for analyzing diverse HTS data.
- To provide a user-friendly platform for hit identification and systems biology interpretation across various screening modalities.
Main Methods:
- HiTSeekR integrates raw data processing, network enrichment, and target generation for multiple HTS types.
- The software supports chemical compound, siRNA, CRISPR/Cas9, and microRNA screens.
Main Results:
- Demonstrated HiTSeekR's utility in three diverse use cases: genome-wide RNAi for TNFα modulators, combined siRNA/miRNA screen for vorinostat resistance, and small compound screen for KRAS synthetic lethality.
- Successfully generated novel hypotheses for follow-up experiments from heterogeneous HTS data.
Conclusions:
- HiTSeekR bridges the gap between raw HTS data and actionable biological insights.
- It is the first tool to offer a unified solution for processing, analyzing, and generating wet lab targets from various HTS screen types.
- HiTSeekR is publicly available, promoting wider adoption in drug discovery and systems biology research.
