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Related Experiment Video

Updated: Mar 19, 2026

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The Not4 RING E3 Ligase: A Relevant Player in Cotranslational Quality Control.

Martine A Collart1

  • 1Department of Microbiology and Molecular Medicine, Faculty of Medicine, CMU, University of Geneva, 1 Rue Michel Servet, 1211 Geneva 4, Switzerland.

ISRN Molecular Biology
|June 24, 2016
PubMed
Summary

The Not4 E3 ligase, part of the Ccr4-Not complex, plays a crucial role in cotranslational quality control by targeting proteins and mRNAs for degradation, ensuring protein homeostasis.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The Not4 RING E3 ligase is a conserved subunit of the Ccr4-Not complex.
  • Not4 functions as an ubiquitin ligase, targeting various substrates for ubiquitination.

Purpose of the Study:

  • To characterize the substrates and functions of the Not4 E3 ligase.
  • To investigate the role of Not4 in cotranslational quality control.

Main Methods:

  • Ubiquitination assays
  • Analysis of protein degradation pathways
  • Studies on polysome levels and protein aggregation

Main Results:

  • Not4 targets some substrates for degradation and others for stable monoubiquitination.
  • Not4 is essential for maintaining polysome levels and preventing the accumulation of aberrant proteins.
  • Not4 associates with RNA degradation machinery and proteasome assembly.

Conclusions:

  • Not4 is a key player in cotranslational quality control, linking translation to degradation pathways.
  • Its function is critical for the quality control of newly synthesized proteins and mRNA surveillance.