Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Organization01:24

Protein Organization

6.2K
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence....
6.2K
Protein Complex Assembly02:41

Protein Complex Assembly

10.5K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.5K
Protein Folding01:25

Protein Folding

7.7K
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
7.7K
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

11.9K
Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA...
11.9K
Ribosomes01:27

Ribosomes

7.2K
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
7.2K
Cotranslational Protein Translocation01:20

Cotranslational Protein Translocation

7.1K
Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
7.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Puf3 contributes to changes in mRNA solubility, translation elongation dynamics at rare arginine codons and loss of protein homeostasis in cells lacking Not4.

RNA (New York, N.Y.)·2026
Same author

Reprogramming neuroblastoma by diet-enhanced polyamine depletion.

Nature·2025
Same author

Corrigendum: Specific roles for the Ccr4-Not complex subunits in expression of the genome.

RNA (New York, N.Y.)·2024
Same author

Dynamic Evolution of Poly-A Tail Lengths Visualized by RNAse H Assay and Northern Blot Using Nonradioactive Probes in Yeast.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Membrane-associated mRNAs: A Post-transcriptional Pathway for Fine-turning Gene Expression.

Journal of molecular biology·2024
Same author

Reprogramming neuroblastoma by diet-enhanced polyamine depletion.

bioRxiv : the preprint server for biology·2024

Related Experiment Video

Updated: May 29, 2025

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions
07:03

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

Published on: December 23, 2022

2.8K

Assembly in action: Protein structure orchestrates assembly pathway, and intertwining defines co-translational

Martine A Collart1, Olesya O Panasenko2

  • 1Department of Microbiology and Molecular Medicine, Institute of Genetics and Genomics Geneva, Geneva, Switzerland.

Molecular Cell
|February 7, 2025
PubMed
Summary

This study investigates protein assembly in living cells, revealing that a protein's 3D structure dictates its assembly pathway during translation. This finding impacts our understanding of protein folding and cellular processes.

More Related Videos

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
05:58

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Published on: July 17, 2019

10.9K
Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach
09:57

Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach

Published on: December 17, 2016

6.6K

Related Experiment Videos

Last Updated: May 29, 2025

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions
07:03

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

Published on: December 23, 2022

2.8K
Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
05:58

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Published on: July 17, 2019

10.9K
Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach
09:57

Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach

Published on: December 17, 2016

6.6K

Area of Science:

  • Cell Biology
  • Biochemistry
  • Structural Biology

Background:

  • Protein assembly is crucial for cellular function.
  • The extent and rules governing co-translational assembly remain incompletely understood.

Purpose of the Study:

  • To investigate the extent of co-translational protein assembly in living cells.
  • To identify the rules governing protein assembly during translation.
  • To understand the role of three-dimensional protein structure in dictating assembly pathways.

Main Methods:

  • Utilized advanced imaging techniques to observe protein assembly in real-time within living cells.
  • Employed biochemical assays to analyze protein interactions and folding intermediates.
  • Applied computational modeling to correlate protein structure with assembly dynamics.

Main Results:

  • Demonstrated significant co-translational assembly of proteins within the cellular environment.
  • Identified specific structural features that predict and direct protein assembly pathways.
  • Established a direct link between a protein's three-dimensional conformation and its assembly trajectory.

Conclusions:

  • The three-dimensional structure of a protein is a primary determinant of its co-translational assembly pathway.
  • Understanding these structural rules provides insights into protein biogenesis and cellular organization.
  • This work advances the field of protein folding and assembly in vivo.