Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

4.4K
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
4.4K
Structure of Cadherins01:25

Structure of Cadherins

5.1K
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
5.1K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

3.9K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.9K
Catenins01:23

Catenins

3.2K
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
3.2K
Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

4.1K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
4.1K
Desmosomes01:05

Desmosomes

8.9K
The term desmosome derives from the Greek words "desmo" and "soma" meaning "adhesion bodies." This structure was first observed during the late 1800s and described as small, dense nodules in the epidermis. Desmosomes are button-like structures that help form an interlinked network of intermediate filaments across the cells. These junctions are  essential to hold cells together under mechanical stress and to maintain tissue integrity. Desmosomes are multi-protein...
8.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SbHsp70 overexpression enhances drought and salinity tolerance in wheat through improved cellular stability and stress-associated structural adaptations.

Frontiers in plant science·2026
Same author

In vivo single-cell RNA metabolic labeling resolves early transcriptional responders in the regenerating zebrafish heart.

Nature communications·2026
Same author

Generation of a prop1 knock-in zebrafish enables single-cell transcriptomics of early pituitary development.

Endocrinology·2026
Same author

Two distinct modes of Vgll4-mediated Tead regulation control organ size in zebrafish.

Communications biology·2026
Same author

Piezo1-mediated mechanohydraulic control of cell volume drives cardiac morphogenesis.

Science advances·2026
Same author

Time-resolved single-cell transcriptomics maps zebrafish heart development.

Cell reports·2026

Related Experiment Video

Updated: Mar 19, 2026

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos
08:57

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

Published on: July 27, 2022

2.2K

N-cadherin relocalization during cardiac trabeculation.

Anoop V Cherian1, Ryuichi Fukuda1, Sruthy Maria Augustine1

  • 1Department of Developmental Genetics, Max Planck Institute for Heart and Lung Research, 61231 Bad Nauheim, Germany.

Proceedings of the National Academy of Sciences of the United States of America
|June 25, 2016
PubMed
Summary

During zebrafish heart development, N-cadherin (Cdh2) transitions from an even distribution to punctate clusters on cardiomyocytes. This remodeling, crucial for cell delamination, requires cardiac contractility and Erb-b2 receptor tyrosine kinase 2 (Erbb2) activity.

Keywords:
Cdh2-EGFPErbb2 signalingN-cadherinheart developmenttrabeculation

More Related Videos

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart
06:51

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

Published on: August 10, 2018

8.9K
Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart
10:56

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart

Published on: March 26, 2015

22.2K

Related Experiment Videos

Last Updated: Mar 19, 2026

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos
08:57

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

Published on: July 27, 2022

2.2K
Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart
06:51

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

Published on: August 10, 2018

8.9K
Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart
10:56

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart

Published on: March 26, 2015

22.2K

Area of Science:

  • Cardiovascular Biology
  • Developmental Biology
  • Cell Adhesion

Background:

  • Cardiac trabeculation involves cardiomyocyte delamination and requires coordinated remodeling of cell adhesion junctions.
  • N-cadherin (Cdh2) is a key cell adhesion molecule implicated in cardiac development.

Purpose of the Study:

  • To investigate the spatiotemporal distribution of N-cadherin (Cdh2) during zebrafish cardiac trabeculation.
  • To determine the role of cardiac contractility and Erb-b2 receptor tyrosine kinase 2 (Erbb2) in Cdh2 localization dynamics.

Main Methods:

  • Utilized a Cdh2-EGFP fusion protein expressed under the zebrafish cdh2 promoter to visualize Cdh2 localization.
  • Employed time-lapse imaging of beating zebrafish hearts.
  • Used a Cdh2 tandem fluorescent protein timer transgenic line to track Cdh2 dynamics.
  • Investigated the effects of inhibiting cardiac contractility and Erbb2 function.

Main Results:

  • Initially, Cdh2-EGFP showed an even distribution along cardiomyocyte lateral sides, evolving into punctate clusters.
  • Cdh2-EGFP also localized to the basal side of cardiomyocytes in the compact layer and on delaminating cells.
  • Cardiac contractility was essential for the transition from even Cdh2 distribution to punctate formation.
  • Cdh2-EGFP molecules were observed to move from lateral to basal cell membrane sides, a process dependent on Erbb2.

Conclusions:

  • Cardiac contractility drives the dynamic redistribution of N-cadherin during trabeculation.
  • Erb-b2 receptor tyrosine kinase 2 (Erbb2) signaling is critical for N-cadherin relocalization to the basal side of cardiomyocytes.
  • These findings elucidate the molecular mechanisms governing cell adhesion remodeling during heart development.