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Bacteriophage infections of microbiota can lead to leaky gut in an experimental rodent model

George Tetz1, Victor Tetz1

  • 1Human Microbiology Institute, 303 5th Avenue, Suite 2012, New York, NY 10016 USA.

Gut Pathogens
|June 25, 2016
PubMed

Insights

Bacteriophages, viruses that infect bacteria, may increase intestinal permeability. This study found that bacteriophage cocktail treatment in rats led to higher gut permeability and circulating immune complexes, suggesting a novel link between bacteriophages and gut health.

Area of Science:

  • Microbiology
  • Gastroenterology
  • Immunology

Background:

  • Increased intestinal permeability and microbial translocation are linked to various diseases.
  • The gut microbiota's role in intestinal permeability is a key research area.
  • Bacteriophages are viruses that infect bacteria and are being explored for therapeutic uses.

Purpose of the Study:

  • To investigate if bacteriophage cocktail treatment can directly induce increased intestinal permeability.
  • To examine host alterations and gut barrier function following bacteriophage administration.

Main Methods:

  • Rats were administered a bacteriophage cocktail for 10 days.
  • Intestinal permeability was assessed using the lactulose:mannitol ratio.
  • Levels of circulating immune complexes were measured.

Main Results:

  • Rats exhibited weight loss, ruffled fur, and reduced activity post-treatment.
  • A significant increase in the lactulose:mannitol ratio was observed, indicating heightened intestinal permeability.
  • Elevated levels of circulating immune complexes were detected.

Conclusions:

  • Bacteriophage cocktail treatment can induce increased intestinal permeability in rats.
  • Bacteriophages may influence gut microbiota composition and function, impacting gut barrier integrity.
  • This study highlights a potential mechanism by which bacteriophages could affect host health via the gut.
Keywords:
BacteriophageIntestinal permeabilityMicrobiota

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