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A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth
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Genetic tools for identifying and manipulating fibroblasts in the mouse
Jessica M Swonger1, Jocelyn S Liu1, Malina J Ivey1
1Departments of Medicine and Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI 96813, USA.
Differentiation; Research in Biological Diversity
|June 26, 2016
Summary
New mouse genetic models help researchers study fibroblast behavior and fibrosis mechanisms in vivo. This review details available tools and their applications for understanding tissue-resident fibroblasts.
Area of Science:
- Fibrosis research
- Cell biology
- Genetics
Background:
- Fibroblasts are crucial in tissue repair and fibrosis.
- Understanding fibroblast heterogeneity is key to studying fibrotic diseases.
- Existing research relies on various in vivo models.
Purpose of the Study:
- To review current mouse genetic models for studying fibroblasts.
- To highlight models applicable to fibrotic organs.
- To discuss the utility and limitations of these genetic tools.
Main Methods:
- Review of literature on mouse genetic models for fibroblast research.
- Categorization of models based on reporter systems (e.g., colorimetric, Cre-lox).
- Analysis of model application in fibrotic organ systems (heart, kidney, liver, lung, muscle).
Main Results:
- Numerous mouse strains are available for fibroblast tracking and manipulation.
- Colorimetric reporters and Cre-expressing lines are valuable tools.
- These models offer insights into fibroblast roles in fibrosis mechanisms.
- Caveats regarding model specificity and efficiency are discussed.
Conclusions:
- Advanced mouse genetic models are essential for in vivo fibroblast research.
- These tools provide critical insights into fibrosis pathogenesis.
- Careful consideration of model limitations is necessary for accurate interpretation.
