Derivation of Genea057 human embryonic stem cell line

Area of Science:

• Stem Cell Biology
• Reproductive Medicine
• Genomics

Background:

• Human embryonic stem cells (hESCs) are crucial for regenerative medicine and disease modeling.
• Characterization of novel hESC lines is essential for expanding research capabilities.

Purpose of the Study:

• To characterize the newly derived Genea057 human embryonic stem cell line.
• To verify its pluripotent nature and genomic stability.

Main Methods:

• Derivation from an ART blastocyst via ICM outgrowth on inactivated human feeders.
• Karyotyping, CGH, and STR analysis for genomic verification.
• Immunocytochemistry for pluripotency markers (Nanog, Oct4, Tra1-60, SSEA4) and PluriTest analysis.

Main Results:

• Genea057 demonstrated typical pluripotent cell morphology.
• Genomic analysis confirmed a 46, XX karyotype and female allele pattern.
• High expression of pluripotency markers (Nanog 97%, Oct4 81%, Tra1-60 75%, SSEA4 97%) and a PluriTest score of 27.59 were observed.
• The cell line was confirmed to be free of Mycoplasma and contamination.

Conclusions:

• The Genea057 hESC line is a well-characterized, pluripotent, and genomically stable resource.
• This cell line meets rigorous standards for potential use in various biomedical research applications.
• Genea057 represents a valuable addition to the available repertoire of human embryonic stem cell lines.