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Generation of a human control iPSC line with a European mitochondrial haplogroup U background
Teresa Galera1, Francisco Zurita1, Cristina González-Páramos1
1Departamento de Bioquímica, Instituto de Investigaciones Biomédicas "Alberto Sols", Facultad de Medicina, (UAM-CSIC) and Centro de Investigación Biomédica en Red en Enfermedades Raras (CIBERER), Madrid, Spain; Instituto de Investigación Hospital 12 de Octubre ("i+12"), Madrid, Spain.
Abstract:
Human iPSC line N44SV.5 was generated from primary normal human dermal fibroblasts belonging to the European mitochondrial haplogroup U. For this purpose, reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using a non-integrative methodology that involves the use of Sendai virus.
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