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Updated: Mar 18, 2026

In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells
Published on: May 2, 2008
Generation of human embryonic stem cell line expressing a red fluorescent protein: BJNhem20-pCAG-tdTomato
Deeti K Shetty1, Maneesha S Inamdar2
1Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, India.
Abstract:
Human embryonic stem cell line BJNhem20-pCAG-tdTomato was generated using non-viral method. The construct pCAG-tdTomato was transfected using microporation procedure. This fluorescent hESC line can help to study heterogeneity within individual cells in hESC colonies by enabling live tracking of their growth, migration and differentiation properties. This cell line also serves as a resource for additional transgene introduction/knock-out/knock-in generation in a fluorescent background and allows ease of analysis in studies involving cell mixing.
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