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Updated: Mar 18, 2026

Derivation of Human Embryonic Stem Cells by Immunosurgery
Published on: December 13, 2007
Derivation of human embryonic stem cell line Genea022
Biljana Dumevska1, Alexis Bosman1, Robert McKernan1
1Genea Biocells, Sydney, Australia.
Abstract:
The Genea022 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XY karyotype and male allele pattern through CGH and STR analysis. Pluripotency of Genea022 was demonstrated with 84% of cells expressed Nanog, 98% Oct4, 55% Tra1-60 and 97% SSEA4, gave a Pluritest Pluripotency score of 42.95, Novelty of 1.23, demonstrated Alkaline Phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination.
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