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Related Concept Videos

Embryonic Stem Cells00:57

Embryonic Stem Cells

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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Zygotic Development And Stem Cell Formation01:10

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The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
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Maintenance of the ES Cell State01:14

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The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
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Derivation of Human Embryonic Stem Cells by Immunosurgery
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Published on: December 13, 2007

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Derivation of Human Embryonic Stem Cells.

Jeremy M Crook1,2,3, Lucy Kravets4, Teija Peura5

  • 1ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, Innovation Campus, University of Wollongong, Fairy Meadow, New South Wales, 2519, Australia. jcrook@uow.edu.au.

Methods in Molecular Biology (Clifton, N.J.)
|March 30, 2017
PubMed
Summary

This study details a standardized protocol for deriving human embryonic stem cells (hESCs) from embryos. These high-quality hESC lines are crucial for research, drug discovery, and potential cell therapies.

Keywords:
BlastocystDerivationHuman embryonic stem cellsInner cell mass

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Area of Science:

  • Stem Cell Biology
  • Developmental Biology
  • Regenerative Medicine

Background:

  • Embryonic stem cells (ESCs) are fundamental to pluripotent stem cell research and development.
  • ESCs serve as a critical reference for evaluating other pluripotent cells, including induced pluripotent stem cells (iPSCs).
  • There is a continuous demand for consistently derived and quality-controlled ESC lines for both research and clinical applications.

Purpose of the Study:

  • To provide a clear, step-by-step protocol for deriving human embryonic stem cells (hESCs).
  • To establish a method suitable for routine derivation of research-grade and potentially clinical-grade hESC lines.
  • To offer a protocol adaptable for clinical compliance in hESC derivation.

Main Methods:

  • The protocol outlines a detailed, step-by-step procedure for hESC derivation from human embryos.
  • It builds upon established methods for clinical-grade hESC line derivation.
  • Emphasis is placed on standardization for reproducible results.

Main Results:

  • A concise and reproducible protocol for hESC derivation has been successfully developed.
  • The method facilitates the generation of quality-controlled hESC lines.
  • The protocol is designed for routine application and adaptability for clinical use.

Conclusions:

  • The presented protocol offers a standardized approach to hESC derivation, essential for advancing stem cell research.
  • This method supports the generation of valuable hESC lines for cell therapies and drug discovery.
  • The protocol's adaptability makes it a valuable tool for both research and future clinical translation.