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Related Experiment Video

Updated: Mar 18, 2026

Primer-Free Aptamer Selection Using A Random DNA Library

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Aptamer Affinity Maturation by Resampling and Microarray Selection.

Andrew B Kinghorn¹, Roderick M Dirkzwager¹, Shaolin Liang¹

¹School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Pokfulam, Hong Kong SAR China.

Analytical Chemistry

|June 28, 2016

Summary

This summary is machine-generated.

A novel aptamer affinity maturation technique significantly enhances nucleic acid affinity reagents. This method improves aptamer binding by tenfold, enabling broader diagnostic and therapeutic applications.

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Area of Science:

Biotechnology
Molecular Biology
Nucleic Acid Chemistry

Background:

Aptamers are valuable affinity reagents with potential in diagnostics, therapeutics, and proteomics.
Current methods for discovering high-affinity aptamers are limited, hindering their wider application.

Purpose of the Study:

To introduce and validate a novel aptamer enhancement technique called aptamer affinity maturation.
To demonstrate the efficacy of aptamer affinity maturation in improving aptamer affinity.

Main Methods:

Aptamer affinity maturation combines bioinformatic resampling of aptamer sequence data with microarray selection.
This integrated approach navigates the complex aptamer sequence-binding landscape.

Main Results:

Aptamer affinity maturation improved aptamer affinity by an order of magnitude in a single round.
The technique generated novel aptamers with complex adaptations not discoverable by other microarray-based methods.

Conclusions:

Aptamer affinity maturation is a powerful technique for enhancing nucleic acid affinity reagents.
Optimizing aptamer sequences through this method will advance the development of next-generation diagnostic and therapeutic tools.