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Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

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Related Experiment Video

Updated: Mar 3, 2026

A Fluorescence-based Protocol for Preliminary Screening of Protein Synthesis Inhibitors from Natural Sources

A Fluorescence-based Protocol for Preliminary Screening of Protein Synthesis Inhibitors from Natural Sources

Published on: January 27, 2026

Techniques for Screening Translation Inhibitors.

Ilya A Osterman¹, Alexey A Bogdanov², Olga A Dontsova³

¹Department of Chemistry and A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia. osterman@yandex.ru.

Antibiotics (Basel, Switzerland)

|June 28, 2016

Summary

This review covers high-throughput methods for screening translation inhibitors, crucial for developing new antibiotics. Combining screening with mechanism of action identification accelerates antibiotic discovery.

Keywords:

antibiotic high throughput screening ribosome translation

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Last Updated: Mar 3, 2026

A Fluorescence-based Protocol for Preliminary Screening of Protein Synthesis Inhibitors from Natural Sources

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Published on: January 27, 2026

An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics

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Published on: September 15, 2020

Quantitative Immunofluorescence to Measure Global Localized Translation

Quantitative Immunofluorescence to Measure Global Localized Translation

Published on: August 22, 2017

Area of Science:

Microbiology
Molecular Biology
Drug Discovery

Background:

Antibiotics often target the translation machinery.
Current antibiotic development involves activity screening followed by mechanism studies.
Identifying molecular targets for new antibiotics is challenging.

Purpose of the Study:

To review methods for screening translation inhibitors.
To emphasize high-throughput screening approaches.
To facilitate the combined screening and mechanism of action identification.

Main Methods:

Review of existing literature on screening methods.
Focus on high-throughput assays for translation inhibition.
Discussion of techniques integrating target identification.

Main Results:

Several high-throughput methods for screening translation inhibitors exist.
Combining screening with mechanism of action studies is feasible.
These approaches can accelerate the discovery of novel antibiotics.

Conclusions:

Efficient screening methods are vital for antibiotic development.
High-throughput techniques combined with target identification streamline the process.
This review provides a resource for researchers in antibiotic discovery.