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PrfH accelerates repair of colicin E3 - damaged translating ribosomes by RtcB2
Tinashe P Maviza1, Nadezhda R Burmistrova2,3, Aleksandr A Grachev4
1Center for Molecular and Cellular Biology, Skolkovo Institute of Science and Technology, Skolkovo, Russia.
Abstract:
Colicin E3 (E3-rRNAse) abolishes protein biosynthesis in bacteria by cleaving 16S rRNA in the decoding centre. The RtcB2-PrfH 16S rRNA repair module prevents cellular death upon exposure to E3-rRNAse in E. coli. Upon overexpression, RtcB2 RNA ligase alone was capable of relieving E. coli growth, which was inhibited by E3-rRNAse. Using in vitro ribosome repair system based on recombinant components, we demonstrated that RtcB2 alone could repair E3-rRNAse cleaved 30S subunits and 70S ribosomes. The peptide chain release factor homolog (PrfH), which is able to hydrolyse peptidyl-tRNA bound by colicin-cleaved ribosomes, boosts RtcB2 ligation efficiency for damaged ribosomes engaged in translation.
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