Methods for Doping Detection

Area of Science:

• Sports Science
• Biochemistry
• Analytical Chemistry

Background:

• The World Anti-Doping Agency (WADA) is expanding its focus beyond small molecules to include larger endogenous molecules for doping detection.
• Implementation of an endocrinological module within the Athlete Biological Passport (ABP) is a key WADA initiative.

Purpose of the Study:

• To discuss the detection of growth hormone (GH) and endogenous anabolic androgenic steroids (EAASs) for doping control.
• To outline current research trends and state-of-the-art detection methods for these substances.
• To introduce microRNAs (miRNAs) as novel biomarkers for anti-doping efforts.

Main Methods:

• Review of historical context and current analytical techniques used in accredited anti-doping laboratories.
• Exploration of advanced methodologies for detecting hormones (GH, EAASs) and microRNAs (miRNAs).

Main Results:

• Established methods for detecting GH and EAASs are in use.
• Emerging research focuses on refining these detection techniques and exploring new biomarkers.
• MicroRNAs (miRNAs) show promise as a new class of doping biomarkers.

Conclusions:

• WADA's strategic shift towards detecting larger endogenous molecules like hormones is advancing anti-doping capabilities.
• State-of-the-art methods and ongoing research are crucial for effective detection of GH, EAASs, and novel biomarkers like miRNAs.
• The integration of endocrinological analysis and new biomarkers will strengthen the Athlete Biological Passport (ABP).