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Molecular diversity and genetic relationships in Secale
1Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Quinta de Prados, 5001-801 Vila Real, Portugal.mmatos@utad.pt.
Journal of Genetics
|June 29, 2016
Summary
This study quantified molecular diversity in rye (Secale spp.) using DNA markers and gene sequencing. ISSR markers proved more informative than RAPDs, revealing significant genetic variability and relationships within the Secale genus.
Area of Science:
- Plant genetics
- Molecular biology
- Agronomy
Background:
- Understanding genetic diversity in rye (Secale spp.) is crucial for crop improvement and conservation.
- Previous studies have explored rye genetics, but comprehensive molecular analyses are ongoing.
Purpose of the Study:
- To quantify molecular diversity and determine genetic relationships among Secale species and Secale cereale cultivars.
- To compare the effectiveness of RAPD and ISSR markers, alongside ScMATE1 gene sequencing, for genetic analysis.
Main Methods:
- Genotyping of 13 rye accessions using 21 RAPD and 16 ISSR primers.
- Sequence analysis of six exons from the ScMATE1 gene.
- Analysis of 435 DNA markers, including 293 polymorphic markers.
Main Results:
- ISSR markers exhibited higher polymorphism and informativeness than RAPD markers.
- Significant genetic variability was observed within the Secale genus, including the ScMATE1 gene.
- Genetic relationships revealed distinct clusters for S. ancestrale, S. kuprijanovii, S. cereale, and S. segetale.
- Secale vavilovii displayed considerable intraspecific variation and suggested it may not be a distinct isolate species.
Conclusions:
- Molecular markers (RAPDs, ISSRs) and gene sequencing (ScMATE1) provide similar insights into rye genetic relationships.
- ISSR markers are highly effective for assessing genetic diversity in rye.
- Secale vavilovii warrants further investigation due to its high intraspecific variability.
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