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Published on: December 31, 2013
Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia
Hiroshi Mihara1, Nobuhiro Suzuki1, Ammar Abdullkader Boudaka1
1Hiroshi Mihara, Nobuhiro Suzuki, Jibran Sualeh Muhammad, Toshiro Sugiyama, Department of Gastroenterology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan.
Aim:
To explore the expression of transient receptor potential vanilloid 4 (TRPV4) and its physiological meaning in mouse and rat gastric epithelia.
Methods:
RT-PCR and immunochemistry were used to detect TRPV4 mRNA and protein expression in mouse stomach and a rat normal gastric epithelial cell line (RGE1-01), while Ca(2+)-imaging and electrophysiology were used to evaluate TRPV4 channel activity. ATP release was measured by a luciferin-luciferase assay. Gastric emptying was also compared between WT and TRPV4 knockout mice.
Results:
TRPV4 mRNA and protein were detected in mouse tissues and RGE1-01 cells. A TRPV4-specific agonist (GSK1016790A) increased intracellular Ca(2+) concentrations and/or evoked TRPV4-like current activities in WT mouse gastric epithelial cells and RGE1-01 cells, but not TRPV4KO cells. GSK1016790A or mechanical stimuli induced ATP release from RGE1-01 cells while TRPV4 knockout mice displayed delayed gastric emptying in vivo.
Conclusion:
TRPV4 is expressed in mouse and rat gastric epithelium and contributes to ATP release and gastric emptying.
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