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LED-Based In Vitro Screening for Assessing Photoactivable Molecules in Bacterial Photodynamic Inactivation

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Spray layer-by-layer films for photodynamic inactivation.

Kevin F Dos Santos¹, Romário J da Silva¹, Karla B Romio¹

¹Grupo de Materiais Nanoestruturados, Universidade Federal de Mato Grosso, Barra do Garças, Mato Grosso, Brazil.

Photodiagnosis and Photodynamic Therapy

|June 30, 2016

Summary

This study introduces a new method for inactivating Candida albicans using ultraviolet light and acridine orange films. The approach effectively kills fungi, showing potential for medical device decontamination.

Keywords:

Acridine orange Atomic force microscopy Fluorescence microscopy UV light

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An In Vitro Approach to Photodynamic Therapy

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Area of Science:

Microbiology
Photochemistry
Materials Science

Background:

A novel photodynamic inactivation (PDI) strategy for Candida albicans is presented.
The method utilizes ultraviolet (UV) light at 254nm combined with layer-by-layer (LbL) films of acridine orange.

Purpose of the Study:

To evaluate the efficacy of UV-PDI combined with acridine orange LbL films for Candida albicans inactivation.
To explore the potential of this method as an alternative antifungal strategy.

Main Methods:

Candida albicans were immobilized on quartz substrates.
LbL films of acridine orange were sprayed onto the immobilized fungi.
Cell viability assays, fluorescence microscopy, and atomic force microscopy were employed for analysis.

Main Results:

Photodynamic inactivation resulted in significant cell death of Candida albicans.
Increased numbers of acridine orange film layers correlated with enhanced fungal cell death.
Microscopy confirmed cell death and indicated damage to the fungal cell wall.

Conclusions:

The developed UV-PDI method is a promising alternative for Candida albicans inactivation.
This approach holds potential for applications in clinical settings, including the decontamination of medical devices.