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Updated: Mar 18, 2026
Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
Published on: November 14, 2017
Estimation and Preparation of the Hypervariable Regions I/II Templates for Mitochondrial DNA Typing From Human Bones
Thien Ngoc Le1, Hieu Van Phan, Anh Tuan Mai Dang
1From the Departments of *Molecular Biology and †Pathology, Forensic Medicine Center; and ‡Molecular Biology Lab, Department of Genetics, Faculty of Biology and Biotechnology, VNUHCM-University of Science, Ho Chi Minh, Vietnam.
Abstract:
A method was designed for estimating and sequencing of mitochondrial DNA (mtDNA) that effectively and more quickly provides a complete mtDNA profile. In this context, we have developed this novel strategy for typing mtDNA from 10 bones and teeth remains (3 months to 44 years). The quantification of mtDNA was achieved by singleplex real-time polymerase chain reaction of the hypervariable region I fragment (445 bp) and hypervariable region II fragment (617 bp). Combined with the melting curve analysis, we have determined as little as 10 pg of mtDNA template that is suitable for sequence analysis. Furthermore, quantitative polymerase chain reaction products were directly used for following step of mtDNA typing by Sanger sequencing. This method allows the profile to be completely provided for faster human identification.
