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Clonal allele loss in gastrointestinal cancers
M F Fey1, C Hesketh, J S Wainscoat
1Department of Haematology, John Radcliffe Hospital, Headington, Oxford, UK.
British Journal of Cancer
|May 1, 1989
Summary
This study investigated chromosomal losses in gastrointestinal cancers. Colorectal cancers frequently showed allele losses on chromosomes 17p and 5q, while gastric cancers displayed losses on chromosomes 1q and 12q.
Area of Science:
- Genetics
- Oncology
- Molecular Biology
Background:
- Chromosomal abnormalities are common in cancer.
- Specific chromosomal regions are frequently altered in gastrointestinal carcinomas.
Purpose of the Study:
- To identify specific chromosomal regions with allele losses in gastrointestinal carcinomas.
- To compare chromosomal loss patterns between colorectal and gastric cancers.
Main Methods:
- Screening of 52 gastrointestinal carcinomas using DNA probes for hypervariable regions.
- Analysis of clonal allele losses on chromosomes 1, 5, 7, 12, 16, and 17.
- Assessment of loss of constitutional heterozygosity at specific loci.
Main Results:
- Colorectal cancers frequently exhibited loss of heterozygosity on chromosome 17p (24/35 cases) and 5q (9/31 cases).
- Allele loss at 5q was strongly associated with loss at 17p in colorectal cancers.
- Gastric cancers showed frequent loss of heterozygosity at 1q (5/10 cases) and 12q (6/11 cases).
Conclusions:
- Gastrointestinal tumors consistently display chromosomal losses.
- The specific chromosomal loci affected by allele loss differ between gastric and colorectal cancers.
- These findings contribute to understanding the distinct genetic landscapes of different gastrointestinal malignancies.