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Evolutionary Diversity of Suid Herpesvirus 1 Based on Ul44 Partial Sequences
Antônio Augusto Fonseca1, Marcelo Fernandes Camargos, Aline Aparecida Silva Barbosa
1Laboratório de Biologia Molecular, Lanagro/MG, Pedro Leopoldo, Brazil.
Intervirology
|July 1, 2016
Summary
Suid herpesvirus 1's Ul44 gene evolution differs between domestic and wild swine, with distinct selective pressures. While Ul44 is key for molecular evolution studies, it offers limited biological insights.
Area of Science:
- Virology
- Molecular Evolution
- Phylogenetics
Background:
- Suid herpesvirus 1 (SuHV-1) is a significant pathogen in swine populations.
- Understanding the evolutionary dynamics of SuHV-1 is crucial for disease management and control.
Purpose of the Study:
- To investigate the evolutionary patterns and population dynamics of SuHV-1 using partial Ul44 (glycoprotein C) gene sequences.
- To compare the evolution of SuHV-1 in different host types (domestic vs. wild swine) and time periods.
Main Methods:
- Phylogenetic analyses were performed using MrBayes and jModelTest to determine optimal models.
- Phylogenetic networks were constructed with SplitsTree 4.0.
- Phylogeographic data were generated using the BEAST program.
- Replication kinetics and serum neutralization tests were conducted on different viral strains.
Main Results:
- Partial Ul44 sequences from domestic and wild swine formed distinct phylogenetic clades.
- Different selective pressures were observed acting on Ul44 sequences depending on the host.
- No significant differences in replication kinetics or serum neutralization were found between tested strains.
- The Ul44 gene serves as a valuable marker for molecular evolution and epidemiological studies of SuHV-1.
Conclusions:
- The evolution of SuHV-1 is complex, with distinct genetic groups potentially evolving at different rates.
- Host species significantly influences the evolutionary trajectory and selective pressures on the SuHV-1 Ul44 gene.
- While informative for evolutionary and epidemiological research, the Ul44 gene provides limited insights into the virus's biological characteristics.
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