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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Related Experiment Video

Updated: Mar 18, 2026

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

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CRISPR-Cas9-Guided Genome Engineering in C. elegans.

Hyun-Min Kim1, Monica P Colaiácovo1

  • 1Department of Genetics, Harvard Medical School, Boston, Massachusetts.

Current Protocols in Molecular Biology
|July 2, 2016
PubMed
Summary
This summary is machine-generated.

This study details a CRISPR-Cas9 protocol for precise genome editing in C. elegans. It covers cloning and germline delivery of Cas9, sgRNA, and repair templates for enhanced genetic engineering.

Keywords:
C. elegansCRISPRCRISPR-CasCas9genome editinggenome engineering

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A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • The CRISPR-Cas9 system enables efficient and targeted genome editing across diverse organisms.
  • CRISPR-Cas9 approaches enhance genome engineering by utilizing DNA double-strand break repair pathways: non-homologous end joining and homologous recombination.

Purpose of the Study:

  • To describe a detailed protocol for Cas9-mediated genome editing in the nematode C. elegans.
  • To outline methods for cloning single guide RNA (sgRNA) and repair templates.
  • To present injection techniques for delivering CRISPR-Cas9 components into the C. elegans germline.

Main Methods:

  • Cas9-mediated genome editing.
  • Single guide RNA (sgRNA) and repair template cloning.
  • Microinjection of Cas9, sgRNAs, and repair template DNA into C. elegans germline.

Main Results:

  • Successful implementation of a CRISPR-Cas9 protocol for C. elegans genome editing.
  • Efficient delivery of necessary components for targeted genetic modification.
  • Established a reproducible method for germline editing in C. elegans.

Conclusions:

  • The described protocol provides a robust method for CRISPR-Cas9 mediated genome engineering in C. elegans.
  • This technique facilitates targeted genetic modifications, advancing research in nematode biology.
  • The protocol supports the use of both non-homologous end joining and homologous recombination pathways for genome editing.