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Related Experiment Video

Updated: Mar 18, 2026

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
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TFEB regulates PER3 expression via glucose-dependent effects on CLOCK/BMAL1.

Wenwen Luo1, Shumin Ma1, Yunzhi Yang1

  • 1Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China.

The International Journal of Biochemistry & Cell Biology
|July 5, 2016
PubMed
Summary

Transcription factor EB (TFEB) directly regulates the PER3 gene, a key component of the mammalian circadian clock. This discovery reveals a novel link between metabolism and the body's internal timing mechanisms.

Keywords:
BMAL1CLOCKCircadian rhythmPER3TFEB

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Area of Science:

  • Chronobiology
  • Molecular Biology
  • Metabolism

Background:

  • Metabolites are known to influence circadian rhythms by affecting clock genes.
  • The involvement of PER3 in metabolic networks suggests potential direct roles for metabolic regulators in the mammalian clock.

Purpose of the Study:

  • To investigate the role of the transcription factor EB (TFEB) in the mammalian circadian clock mechanism.
  • To determine if TFEB directly regulates circadian clock genes, specifically PER3.

Main Methods:

  • Investigated TFEB's regulation of PER3 using molecular biology techniques.
  • Examined TFEB's interaction with the CLOCK/BMAL1 complex.
  • Assessed the impact of glucose on the TFEB/CLOCK/BMAL1 complex.

Main Results:

  • TFEB expression exhibits circadian rhythms in the liver.
  • TFEB directly regulates PER3 expression via the CLOCK/BMAL1 complex.
  • TFEB physically interacts with CLOCK/BMAL1, enhancing its transcriptional activity.
  • The TFEB/CLOCK/BMAL1 complex is sensitive to glucose levels.

Conclusions:

  • TFEB plays a significant role in the mammalian circadian clock.
  • TFEB acts as a direct regulator of PER3, linking metabolic status to circadian rhythmicity.