Development and validation of an event-specific detection method for WYN029GmA soybean based on TaqMan qPCR

This study aimed to establish an event-specific detection method for the genetically modified soybean WYN029GmA. WYN029GmA is a glyphosate-resistant transgenic soybean carrying the mam79 epsps gene, independently developed in China, and has been granted the China Agricultural Genetically Modified Organism Safety Certificate (Production Application). Based on the junction region between the exogenous insertion sequence and the flanking genomic sequences of WYN029GmA, multiple sets of primers and probes were designed. After screening, WYN029LB-QF4/QR2/QP2 was selected as the optimal primer-probe combination, and the amplified fragment length is 95 bp. The method was systematically evaluated by a series of tests such as specificity, dynamic range, accuracy, and robustness, and its practical applicability was evaluated through an interlaboratory validation involving eight laboratories. The results demonstrated that this method showed good specificity and repeatability across different laboratories, and could meet the technical requirements of qualitative and quantitative detection of WYN029GmA. This method provides reliable technical support for the traceability, safety supervision, and labeling management of WYN029GmA soybean and its derivatives.